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Quality analysis 
FastQC 
Prinseq 

 

Metagenome assembly 
Velvet  http://www.ebi.ac.uk/~zerbino/velvet/ 

Celera http://www.cbcb.umd.edu/research/assembly.shtml#software 

Metasim(Simulator-used to compare predictions) http://ab.inf.unituebingen.de/software/metasim/welcome.html#Download 

Euler  http://nbcr.sdsc.edu/euler/ 

JAZZ 

 

Gene calling 
genemark.hmm(using HMM models to identify genes)  http://exon.gatech.edu/GeneMark/metagenome/Prediction/

MetaGeneMark  
FragGeneScan  
MetaGeneAnnotator 
Orphelia  
Metagene 

 

Microbial diversity Analysis

MLST(http://www. mlst.net)  http://www.mlst.net/

MOTHUR  http://www.mothur.org/

EstimateS  http://viceroy.eeb.uconn.edu/EstimateS/

QIIME  http://qiime.org/install/virtual_box.html

PHACCS  http://phaccs.sourceforge.net/

 

Binning 
Composition based binning 
TETRA  http://www.megx.net/tetra/index.html

Phylopathia  http://cbcsrv.watson.ibm.com/phylopythia.html

Sequence similiarity based binning 
MEGAN  http://ab.inf.uni-tuebingen.de/software/megan/

CARMA  http://www.cebitec.uni-bielefeld.de/brf/carma/carma.html

Phymm  http://www.cbcb.umd.edu/software/phymm/

 

Functional Annotation 
MEX(Motif Extraction)  http://adios.tau.ac.il/SPMatch/

MG-RAST  http://metagenomics.anl.gov/

RAMMCAP(Rapid analysis of Multiple Metagenomes with Clustering and Annotation Pipeline) 
http://weizhong-lab.ucsd.edu/rammcap/cgi-bin/rammcap.cgi

 

Comparitive Metagenomics 
MEGAN  http://metagenomics.anl.gov/

MG-RAST  http://metagenomics.anl.gov/

Camera  http://camera.calit2.net/#

ShotgunFunctionalizeR  http://shotgun.math.chalmers.se/

UniFrac  http://bmf.colorado.edu/unifrac/

MetaStats  http://metastats.cbcb.umd.edu/detection.html

Galaxy  https://main.g2.bx.psu.edu/u/aun1/w/metagenomic-analysis

MetaMine  http://www.megx.net/metamine/

MetaLook  http://www.megx.net/metalook/index.php

IMG/M  http://img.jgi.doe.gov/cgi-bin/m/main.cgi

 

Mapping to reference genome

Bowtie  http://bowtie-bio.sourceforge.net/index.shtml

BWA  http://bio-bwa.sourceforge.net/

SOAPZ 

MCQ 

SURPI: Pipeline for pathogen identification from complex metagenomic NGS data.

http://chiulab.ucsf.edu/surpi/

http://genome.cshlp.org/content/early/2014/05/16/gr.171934.113.full.pdf+html

 

Online tools for NGS data analysis

CONTIGuator: bacterial genomes finishing tool http://contiguator.sourceforge.net/

parallel Meta see

Sort-Items 

PANGEA 

http://www.ebi.ac.uk/metagenomics

AmphoraNet: metagenomic analysis webserver

AmphoraVizu: Chart visualization webserver for metagenomics analysis tools AMPHORA2 and AmphoraNet

FANTOM: functional and taxonomic analysis of metagenomes

MetaPhlAn: metagenomic phylogenetic analysis

 

Commercial 
CLC bio genomic workbench 
ERA-7 

 

 

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